Part:BBa_K4369001

Complete K. xylinus cellulose operon (adjusted for E. coli)

This part contains the complete cellulose operon from Komagataeibacter xylinus, and is codon optimized for E. coli. For the cellulose production in K. xylinus 4 enzymes are needed which synthesize and structure the polysaccharide. The main enzyme is the cellulose synthase, which consists of 4 subunits acsA-D. To help getting the cellulose-fibres in the right arrangement an endo-beta-1,4-glucanase and cellulase-complementing protein is needed, additionally a beta-glucosidase and endoglucanase can hydrolyse cellulose fibres, when assembled in the wrong arrangements. BBa_K4369001 contains parts from other iGEM teams and combines them to make it possible to synthesize bacterial cellulose as it is in K. xylinus. Behind every subunit/protein in this part we added a His-tag, to be able to detect them with affinity protein purification.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 4008
  Illegal BglII site found at 4722
  Illegal BglII site found at 6645
  Illegal BglII site found at 8716
  Illegal BglII site found at 11278
  Illegal BamHI site found at 1107
  Illegal BamHI site found at 3018
  Illegal BamHI site found at 6012
  Illegal BamHI site found at 8785
  Illegal BamHI site found at 12591
  Illegal XhoI site found at 1760
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 223
  Illegal NgoMIV site found at 11943
  Illegal NgoMIV site found at 12995
  Illegal AgeI site found at 3994
  Illegal AgeI site found at 4071
  Illegal AgeI site found at 4627
  Illegal AgeI site found at 5428
  Illegal AgeI site found at 5520
  Illegal AgeI site found at 5626
  Illegal AgeI site found at 7657
  
• 1000
  COMPATIBLE WITH RFC[1000]